heart fibroblasts hcf Search Results


human cardiac fibroblasts  (PromoCell)
97
PromoCell human cardiac fibroblasts
Human Cardiac Fibroblasts, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cardiac fibroblasts  (Cell Applications Inc)
95
Cell Applications Inc human cardiac fibroblasts
Human Cardiac Fibroblasts, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cardiac fibroblasts - by Bioz Stars, 2026-03
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primary cardiac fibroblasts  (PromoCell)
95
PromoCell primary cardiac fibroblasts
Primary Cardiac Fibroblasts, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary cardiac fibroblasts - by Bioz Stars, 2026-03
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hcf growth medium  (PromoCell)
97
PromoCell hcf growth medium
Cardiomyocyte-derived exosomes regulate cardiac fibroblast to myofibroblast transformation. ( A ) Schematic diagram for studying the effects of human <t>cardiomyocyte</t> <t>(HCM)-derived</t> exosome uptake by human cardiac fibroblasts <t>(HCF)</t> using early endosome antigen 1(EEA1) staining. ( B ) Immunocytochemistry and confocal microscopy identified the time-dependent intake and regulation of HCM-derived exosomes by EEA1 in HCFs. Nuclear DNA, exosomes, and EEA1 were labeled with 4′,6-diamidino-2-phenylindole (DAPI, blue), Dil dye (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine, red), and GFP fluorescence dye (green), respectively. Data are representative of three independent experiments ( n = 3). ( C ) HCFs were treated with or without HCM-derived exosomes, HCM+MCT-derived exosomes, or rTHBS2 (THBS2 recombinant protein, 1 μg/mL) for 48 h to assess cardiomyocyte-derived exosomal THBS2-mediated fibroblast to myofibroblast transformation. Expression of the myofibroblast marker proteins, α-SMA, β-catenin, fibronectin, and vimentin was examined by immunocytochemistry and confocal microscopy with Imaris software for confocal image 3D reconstruction. ( D , E ) Myofibroblast marker protein expression in myocardial tissues of MCT-induced PAH mice was examined by western blotting and immunohistochemistry. Data are representative of three independent experiments ( n = 3), and values are expressed as median and interquartile range. Mann–Whitney U-test was used to compare two independent groups (* p < 0.05).
Hcf Growth Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hcf growth medium/product/PromoCell
Average 97 stars, based on 1 article reviews
hcf growth medium - by Bioz Stars, 2026-03
97/100 stars
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human cardiac fibroblasts-adult  (ScienCell)
90
ScienCell human cardiac fibroblasts-adult
Cardiomyocyte-derived exosomes regulate cardiac fibroblast to myofibroblast transformation. ( A ) Schematic diagram for studying the effects of human <t>cardiomyocyte</t> <t>(HCM)-derived</t> exosome uptake by human cardiac fibroblasts <t>(HCF)</t> using early endosome antigen 1(EEA1) staining. ( B ) Immunocytochemistry and confocal microscopy identified the time-dependent intake and regulation of HCM-derived exosomes by EEA1 in HCFs. Nuclear DNA, exosomes, and EEA1 were labeled with 4′,6-diamidino-2-phenylindole (DAPI, blue), Dil dye (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine, red), and GFP fluorescence dye (green), respectively. Data are representative of three independent experiments ( n = 3). ( C ) HCFs were treated with or without HCM-derived exosomes, HCM+MCT-derived exosomes, or rTHBS2 (THBS2 recombinant protein, 1 μg/mL) for 48 h to assess cardiomyocyte-derived exosomal THBS2-mediated fibroblast to myofibroblast transformation. Expression of the myofibroblast marker proteins, α-SMA, β-catenin, fibronectin, and vimentin was examined by immunocytochemistry and confocal microscopy with Imaris software for confocal image 3D reconstruction. ( D , E ) Myofibroblast marker protein expression in myocardial tissues of MCT-induced PAH mice was examined by western blotting and immunohistochemistry. Data are representative of three independent experiments ( n = 3), and values are expressed as median and interquartile range. Mann–Whitney U-test was used to compare two independent groups (* p < 0.05).
Human Cardiac Fibroblasts Adult, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cardiac fibroblasts-adult - by Bioz Stars, 2026-03
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Image Search Results


Cardiomyocyte-derived exosomes regulate cardiac fibroblast to myofibroblast transformation. ( A ) Schematic diagram for studying the effects of human cardiomyocyte (HCM)-derived exosome uptake by human cardiac fibroblasts (HCF) using early endosome antigen 1(EEA1) staining. ( B ) Immunocytochemistry and confocal microscopy identified the time-dependent intake and regulation of HCM-derived exosomes by EEA1 in HCFs. Nuclear DNA, exosomes, and EEA1 were labeled with 4′,6-diamidino-2-phenylindole (DAPI, blue), Dil dye (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine, red), and GFP fluorescence dye (green), respectively. Data are representative of three independent experiments ( n = 3). ( C ) HCFs were treated with or without HCM-derived exosomes, HCM+MCT-derived exosomes, or rTHBS2 (THBS2 recombinant protein, 1 μg/mL) for 48 h to assess cardiomyocyte-derived exosomal THBS2-mediated fibroblast to myofibroblast transformation. Expression of the myofibroblast marker proteins, α-SMA, β-catenin, fibronectin, and vimentin was examined by immunocytochemistry and confocal microscopy with Imaris software for confocal image 3D reconstruction. ( D , E ) Myofibroblast marker protein expression in myocardial tissues of MCT-induced PAH mice was examined by western blotting and immunohistochemistry. Data are representative of three independent experiments ( n = 3), and values are expressed as median and interquartile range. Mann–Whitney U-test was used to compare two independent groups (* p < 0.05).

Journal: International Journal of Molecular Sciences

Article Title: miR-29a-3p/THBS2 Axis Regulates PAH-Induced Cardiac Fibrosis

doi: 10.3390/ijms221910574

Figure Lengend Snippet: Cardiomyocyte-derived exosomes regulate cardiac fibroblast to myofibroblast transformation. ( A ) Schematic diagram for studying the effects of human cardiomyocyte (HCM)-derived exosome uptake by human cardiac fibroblasts (HCF) using early endosome antigen 1(EEA1) staining. ( B ) Immunocytochemistry and confocal microscopy identified the time-dependent intake and regulation of HCM-derived exosomes by EEA1 in HCFs. Nuclear DNA, exosomes, and EEA1 were labeled with 4′,6-diamidino-2-phenylindole (DAPI, blue), Dil dye (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine, red), and GFP fluorescence dye (green), respectively. Data are representative of three independent experiments ( n = 3). ( C ) HCFs were treated with or without HCM-derived exosomes, HCM+MCT-derived exosomes, or rTHBS2 (THBS2 recombinant protein, 1 μg/mL) for 48 h to assess cardiomyocyte-derived exosomal THBS2-mediated fibroblast to myofibroblast transformation. Expression of the myofibroblast marker proteins, α-SMA, β-catenin, fibronectin, and vimentin was examined by immunocytochemistry and confocal microscopy with Imaris software for confocal image 3D reconstruction. ( D , E ) Myofibroblast marker protein expression in myocardial tissues of MCT-induced PAH mice was examined by western blotting and immunohistochemistry. Data are representative of three independent experiments ( n = 3), and values are expressed as median and interquartile range. Mann–Whitney U-test was used to compare two independent groups (* p < 0.05).

Article Snippet: Primary human cardiac fibroblasts (HCF; C-12375; PromoCell, Heidelberg, Germany) and primary human cardiac myocytes (HCM; ScienCell Research Laboratories, Carlsbad, CA, USA) were isolated from the ventricles of an adult heart and cultured in a specific culture media, namely, HCF growth medium (C-23010; PromoCell) and HCM medium (ScienCell Research Laboratories) supplemented with 1% penicillin/streptomycin solution (ScienCell Research Laboratories, Carlsbad, CA, USA) and 5% fetal bovine serum.

Techniques: Derivative Assay, Transformation Assay, Staining, Immunocytochemistry, Confocal Microscopy, Labeling, Fluorescence, Recombinant, Expressing, Marker, Software, Western Blot, Immunohistochemistry, MANN-WHITNEY